Reverse Effects of Tetraarsenic Oxide on the Angiogenesis Induced by Nerve Growth Factor in the Rat Cornea

Journal of Veterinary Medical Science

Tchi-Chou NAM¹  Myung-Hyun YOO³  Chang-Hun RHEE²  Na-Young YI¹  Myung-Jin PARK²  Seong-Mok JEONG¹  Jong-Taek KIM³  Ill-Ju BAE⁴  Kang-Moon SEO¹  Man-Bok JEONG¹ 

[1] College of Veterinary Medicine, Seoul National University;Laboratory of Cell Biology, Korea Cancer Hospital;Department of Veterinary Medicine, Kangwon National University;Laboratory of Chonjisan Institute

关键词: As4O6;    As2O3;    antiangiogenesis;    NGF;    rat;   

DOI  :  10.1292/jvms.66.1091

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(6)To compare the antiangiogenic effects of tetraarsenic oxide (As4O6) with those of diarsenic oxide (As2O3) in the rat cornea, rat cornea micropocket assay was conducted to induce angiogenesis by implantation of the pellet contained 1.0 ng of nerve growth factor (NGF). Ten of thirty eyes of Sprague-Dawley rats were randomly assigned to one of three groups, namely, control group (no medication), As2O3 group (50 mg/kg As2O3, PO, s.i.d.), and As4O6 group (50 mg/kg As4O6, PO, s.i.d.). After implantation, the number of new vessels, vessel length and clock hour of neovascularization were examined under the microscope from day 3 to day 7. The area of neovascularization was calculated using a mathematical formula. Although new vessels in control and As2O3 groups were first noticed at day 3, whereas those of As4O6 group were first observed on day 5. The number, length, clock hour of neovascularization and areas of the vessels in As4O6 group showed more significant inhibition than those of control and As2O3 groups from day 5 (P<0.05). However, there were no differences in all parameters between control group and As2O3 group during the entire study period. These results showed that As4O6 had antiangiogenic effects on the new vessels induced by NGF in the rat cornea.

【 授权许可】

Unknown