Biological effects of mitochondrial therapy: preventing development of myocardial infarction and blocking metastatic aggression of B16/F10 melanoma

Cardiometry

article

Oleg I. Kit¹  Elena M. Frantsiyants¹  Alla I. Shikhlyarova¹  Irina V. Neskubina¹  Irina V. Kaplieva¹  Natalia D. Cheryarina¹  Alexandra A. Vereskunova²  Lidia K. Trepitaki¹  Yulia A. Pogorelova¹  Valeria A. Bandovkina¹  Ekaterina I. Surikova¹  Polina S. Kachesova¹  Elena A. Sheiko¹  Inga M. Kotieva¹  Marina A. Gusareva¹  Roza G. Luganskaya¹  Ekaterina S. Bosenko¹ 

[1] National Medical Research Centre of Oncology Russia;Rostov State Medical University Russia

关键词: Mitochondrial therapy;    Mitochondria;    Heart;    Myocardial infarction;    B16/F10 melanoma;    Chronic neurogenic pain;    Male mice;   

DOI  :  10.18137/cardiometry.2022.22.5055

学科分类：环境科学（综合）

来源: Russian New University

【 摘 要 】

The aim is to evaluate the physiological parameters of the efficacy of cardiac mitochondria transplantation in male mice withchronic neurogenic pain and B16/F10 melanoma growth.Materials and methods. Male mice (n=37) of the C57BL/6line were used in the research work. The animals covered experimental groups as follows: mice with chronic neurogenicpain (CNP) + B16/F10 melanoma (n=27); mice with CNB + B16/F10 melanoma + mitochondrial therapy (MC therapy) (n=10).Mitochondria were isolated from the heart of an intact rat withthe use of differential centrifugation. An introduction of mitochondria to mice was carried out daily intraperitoneally at adose of 3.3 mg of protein for 3 weeks.Statistical analysis of the results is carried out with the Statistica10.0 software.Results. On day 21 (week 3) of the experiment, macroscopicallyin the melanoma tissue in the group of animals with MC therapy, there were 2.5 times more necrosis cases than in the groupwithout MC therapy. During the examination of the internal organs, no metastases were detected in the animals treated withMC therapy, while in 100% of the animals without MC therapymetastases were found in the lungs and in 95% of them in thespleen. In the animals received MC therapy, there was no damage to the heart muscle in 75% of the cases, while in the groupof the animals without MC therapy, the presence of lesions inthe form of bruises on the surface of the heart was macroscopically detected in 100% of the animals.Conclusion. Thus, intraperitoneal transplantation of intactheart mitochondria contributed to the prevention of myocardial infarction and metastases to internal organs in the C57BL/6male mice with B16/F10 melanoma growing against the background of chronic neurogenic pain.

【 授权许可】

CC BY