Mitochondrial therapy of melanoma B16/F10, pathophysiological parameters of tumor regression

Cardiometry

article

Oleg I. Kit¹  Elena M. Frantsiyants¹  Alla I. Shikhlyarova¹  Irina V. Neskubina¹  Irina V. Kaplieva¹  Lidia K. Trepitaki¹  Yulia A. Pogorelova¹  Natalia D. Cheryarina¹  Alexandra A. Vereskunova²  Valeria A. Bandovkina¹  Ekaterina I. Surikova¹  Natalia A. Maksimova¹  Inga M. Kotieva¹  Marina A. Gusareva¹  Viktoria V. Pozdnyakova¹ 

[1] National Medical Research Centre of Oncology Russia;Rostov State Medical University Russia

关键词: Mitochondrial therapy;    Mitochondria;    B16/F10 melanoma;    BALB/c Nude mice;   

DOI  :  10.18137/cardiometry.2022.22.5661

学科分类：环境科学（综合）

来源: Russian New University

【 摘 要 】

The aim is to evaluate the pathophysiological parameters ofthe efficacy of liver mitochondrial transplantation in animalswith B16/F10 melanoma.Materials and methods. In our experiment we used femaleand male mice of BALB/c Nude strain (n=28). Experimentalgroups were as follows: the reference group (n=14) with B16/F10 melanoma; the main group (n=14) with B16/F10 melanoma + mitochondrial therapy (MC therapy). Statistical analysis ofresults was carried out with the Statistica 10.0 software.Results. The subcutaneous tumor in the mice of both sexesbecame detectable on day 5 from the time of the tumor inoculation, and the regressive effect produced by MC therapywas recorded in the males beginning with day 8 of the tumorgrowth. At the end of the experiment, on day 22, the differencein the average volumes of the tumor node was reported to be3.2 times, i.e. a significant inhibition of the tumor growth in thegroup of the males with MC therapy was revealed. In the females on day 5 of the tumor growth, differences in the volumeof the tumor focus between the reference group and the groupwith MC therapy were not recorded, however, a statistically significant difference was found in the sex-related comparison ofthe groups of the animals with MC therapy. It was determinedthat in the females with MC therapy, the area of the tumor spotduring that period (5 days) was 1.4 times (p<0.05) less than that in the corresponding group of the males. On day 8, in thefemales completed MC therapy, the tumor has not yet concentrated into a solid structure, but remained as a flat tumor entity,and only by day 12 the tumor has formed from a flat structureinto a volumetric tumor type. As a result, by the end of theexperiment, on day 22, smaller volumes of the tumor nodesremained in the group of females treated with MC therapy, andthe difference with the reference group was 2.7 times (p<0.05).Conclusion. Thus, within the framework of the experiment, ithas been found that the application of mitochondrial therapyusing allogeneic liver mitochondria in the BALB/c Nude micewith B16/F10 melanoma retards the tumor growth in the miceof both sexes.

【 授权许可】

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