In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium

Natural and Engineering Sciences

article

Muhammet Dogan¹ 

[1] Karamanoglu Mehmetbey University, Faculty of Health Sciences, Department of Nutrition and Dietetics

关键词: MS medium;    Shoot regeneration;    Shoot tip explant;    Tissue culture;   

DOI  :  10.28978/nesciences.1098681

学科分类：数学（综合）

来源: Natural and Engineering Sciences

【 摘 要 】

Pogostemon erectus (Dalzell) Kuntze is a valuable aquatic-ornamental plant. This study was designed for the multiplication of P. erectus in a liquid culture medium. In this context, shoot tip explants of P. erectus were placed in Murashige and Skoog (MS) food solutions including Zeatin (ZEA: 0.1-2.4 mg/L) and indole acetic acid (IAA: 1.2 mg/L). In addition, experiments were set up in hormone-free environments as controls. In general, high-frequency results were recorded. 100 % regeneration was determined in the treatment of 0.6 mg/L ZEA + 1.2 mg/L IAA. The best results for shoot count and shoot length were obtained with 0.6 mg/L ZEA + 1.2 mg/L IAA and 0.3 mg/L ZEA + 1.2 mg/L IAA, respectively. Generally, low regeneration values were seen in nutrient solutions using high or low doses of ZEA. Shoots were rooted in MS nutrient media with 0.25 mg/L IAA. Then they were transferred to an aquarium and successfully adapted to external conditions.

【 授权许可】

CC BY