Neuroprotective Effect of Celastrus Paniculatus Seed Extract on Epilepsy and Epilepsy-associated Cognitive Deficits

Basic and Clinical Neuroscience

article

Arti Ralta¹  Ajay Prakash¹  Praveen Kumar_M¹  Rohit Kumar¹  Phulen Sarma¹  Alka Bhatia²  Bikash Medhi¹  Amitava Chakrabarti¹ 

[1] Department of Pharmacology, Postgraduate Institute of Medical Education and Research;Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research

关键词: C. paniculatus;    Hippocampus;    Kindling;    Oxidative stress;    Pentylenetetrazole;   

DOI  :  10.32598/bcn.2021.3154.1

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: Cognitive deficit is one of the common comorbidity accompanying epilepsy. The present study evaluated the effect of Celastrus paniculatus seed extract on seizure severity and cognitive deficit following the pentylenetetrazole (PTZ)-induced chemical kindling model. Methods: PTZ kindling model was developed by daily administration of the sub-convulsive dose of PTZ 30 mg/kg for four weeks. After four weeks of induction, the following treatment, namely sodium valproic acid (SVA) 200 mg/kg, C. paniculatus 500 mgkg, pergolide 2 mg/kg, C. paniculatus (250 mgkg)+ Pergolide (1 mg/kg), and C. paniculatus (250 mgkg)+ SVA (100 mg/kg) were administered 30 minutes prior to PTZ (30 mg/kg) injection for a period of next 14 days. Neurobehavioral parameters, including superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and dopamine levels were assessed and the Morris water maze test (MWM) and Grip strength test (GPS) were performed. Hematoxylin & Eosin (H&E) staining of hippocampal cornu ammonis (CA1), CA2, CA3, dentate gyrus (DG), and frontal cortex was performed. Results: C. paniculatus (500 mg/kg) alone and in combination ( C. paniculatus (250 mgkg)+ pergolide (1 mg/kg) and C. paniculatus (250 mgkg)+ SVA (100 mg/kg)) significantly (p<0.05) reduced the seizure score, mean latency time, and distance traveled in the MWM. However, no significant effect was seen in GPS. Biochemical analysis showed elevated antioxidant markers, namely GSH, CAT, and SOD, and also elevated dopamine levels. C. paniculatus and its combination also significantly (P<0.05) protected against neuronal loss in the hippocampus and frontal cortex evidenced by H&E staining Conclusion: C. paniculatus alone and in combination with other agents may have the potential to treat epilepsy and associated cognitive deficits.

【 授权许可】

Unknown