Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line

Turkish Journal of Pharmaceutical Sciences

article

Ömer Faruk KIRLANGIǹ  Ecem KAYA-SEZGİNER²  Sema ÖREN³  Serap GÜR⁴  Özlem YAVUZ⁵  Taner ÖZGÜRTAŞ⁵ 

[1] Gazi University, Faculty of Medicine, Department of Medical Biochemistry;Ankara University, Faculty of Pharmacy, Department of Biochemistry;University of Health Sciences Türkiye, Gülhane Health Sciences Institute Research Center;Ankara University, Faculty of Pharmacy, Department of Pharmacology;University of Health Sciences Türkiye, Gülhane Training and Research Hospital, Department of Clinical Biochemistry

关键词: Apoptosis;    borax;    colorectal adenocarcinoma;    DLD-1;    5-fluorouracil;   

DOI  :  10.4274/tjps.galenos.2021.29726

来源: Galenos Yayinevi

【 摘 要 】

Objectives: Colorectal cancer (CRC) remains a crucial health problem due to the toxicity of 5-Fluorouracil (5-FU) as first-line chemotherapy agent for treating CRC. The anticancer effects of boron and its compounds have been shown in various cell lines. This study aimed to examine the cytotoxic and apoptotic effects of borax (sodium tetraborate) alone or along with 5-FU on human CRC cells, DLD-1. Materials and Methods: Cytotoxicity and apoptosis were determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, 4’,6-diamidino-2-phenylindole and annexin V/propidium iodide staining. Results: The results showed that combined treatment revealed a significant time- and concentration-dependent cytotoxic effect on DLD-1 cells compared with borax or 5-FU treatment alone. The combination of borax and 5-FU induced a clear increase in the early apoptotic cell percentage, compared to the cells treated with monotherapies. Additionally, a significant increase in condensed and fragmented nuclei was detected in DLD-1 cells treated with the combination treatment compared with borax or 5-FU alone. Conclusion: Our current findings suggest that the combination of borax with 5-FU has a strong cytotoxic and apoptotic effect on the human CRC DLD-1 cells.

【 授权许可】

Unknown