Protective activity of glutamine against bisphenol A-induced nephrotoxicity in rats

Journal of Clinical and Basic Research

article

Enoluomen Ben Ehigiator¹  Elias Adikwu²  Anthony Chiwendu Ifeduba¹ 

[1] Department of Pharmacology and Toxicology, Faculty of Pharmacy, Madonna University Elele;Department of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University

关键词: Bisphenol A;    Kidneys;    Toxicity;    Glutamine;    Rats;   

DOI  :  10.29252/jgbfnm.19.2.23

学科分类：数学（综合）

来源: Akademika As, Akademika Forlag

【 摘 要 】

Background: Bisphenol A (BPA) can impair kidney function via oxidative stress. Glutamine (Gln) is an amino acid withantioxidant and immunomodulatory activities. This study assessed the protective activity of Gln against BPA-inducednephrotoxicity in ratsMethods: Thirty adult male Wistar rats (200-230g) were randomly divided into 6 groups (each containing 5 rats). The rats wereorally treated daily for 60 days as follows: Groups A (Control), B, and C were treated with normal saline (0.2 mL), Gln (80mg/kg), and BPA (50 mg/kg), respectively. Groups D-F were supplemented with 20 mg/kg, 40 mg/kg, and 80 mg/kg of Glnbefore treatment with BPA (50 mg/kg), respectively. Blood samples were collected and serum renal biochemical markers weremeasured. The kidneys were weighed and evaluated for oxidative stress markers and histological changes.Results: The administration of BPA decreased body weight (p<0.01) and increased kidney weight (p<0.01) when compared withthe control group. The BPA-induced alterations in serum renal biochemical markers were accompanied by elevated urea(p<0.001), creatinine (p<0.001), and uric acid levels (p<0.001) as well as decreased electrolytes (p<0.01) when compared withthe control group. Altered kidney oxidative stress markers caused by BPA were marked by a significant decrease in glutathione,catalase, superoxide dismutase, and glutathione peroxidase levels (p<0.001) with a significant increase in malondialdehyde levels(p<0.001) when compared with the control group. Moreover, BPA caused kidney tubular necrosis, widened bowman’s space,collapsed glomerulus, and lipid accumulation. However, supplementation with Gln (20, 40, and 80 mg/kg) significantly reversedthe BPA-induced nephrotoxicity in a dose-dependent manner when compared with the BPA group. Furthermore, different dosesof Gln restored kidney histology.Conclusion: Based on the results, Gln may have clinical protective effect against BPA-associated nephrotoxicity.

【 授权许可】

CC BY-NC