Berberine inhibits RA-FLS cell proliferation and adhesion by regulating RAS/MAPK/FOXO/HIF-1 signal pathway in the treatment of rheumatoid arthritis

Bone & Joint Research

article

Zhiqi Li¹  Meilin Chen¹  Zhaoyi Wang¹  Qiqi Fan¹  Zili Lin¹  Xiaoyu Tao²  Jiarui Wu¹  Zhenquan Liu¹  Ruichao Lin¹  Chongjun Zhao¹ 

[1] Beijing University of Chinese Medicine;Beijing Key Lab for Quality Evaluation of Chinese Materia Medica

关键词: Berberine;    Traditional Chinese medicine;    Rheumatoid arthritis;    rheumatoid arthritis;    fibroblast-like synoviocytes;    MMP-3;    Western blot;    hypoxia;    quantitative polymerase chain reaction;    Enzyme-linked immunosorbent assay;    matrix metalloproteinases (MMPs);    arthritis;    polymerase chain reaction;   

DOI  :  10.1302/2046-3758.122.BJR-2022-0269.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsRheumatoid arthritis (RA) is a common chronic immune disease. Berberine, as its main active ingredient, was also contained in a variety of medicinal plants such as Berberaceae, Buttercup, and Rutaceae, which are widely used in digestive system diseases in traditional Chinese medicine with anti-inflammatory and antibacterial effects. The aims of this article were to explore the therapeutic effect and mechanism of berberine on rheumatoid arthritis.MethodsCell Counting Kit-8 was used to evaluate the effect of berberine on the proliferation of RA fibroblast-like synoviocyte (RA-FLS) cells. The effect of berberine on matrix metalloproteinase (MMP)-1, MMP-3, receptor activator of nuclear factor kappa-Β ligand (RANKL), tumour necrosis factor alpha (TNF-α), and other factors was determined by enzyme-linked immunoassay (ELISA) kit. Transcriptome technology was used to screen related pathways and the potential targets after berberine treatment, which were verified by reverse transcription-polymerase chain reaction (RT-qPCR) and Western blot (WB) technology.ResultsBerberine inhibited proliferation and adhesion of RA-FLS cells, and significantly reduced the expression of MMP-1, MMP-3, RANKL, and TNF-α. Transcriptional results suggested that berberine intervention mainly regulated forkhead box O (FOXO) signal pathway, prolactin signal pathway, neurotrophic factor signal pathway, and hypoxia-inducible factor 1 (HIF-1) signal pathway.ConclusionThe effect of berberine on RA was related to the regulation of RAS/mitogen-activated protein kinase/FOXO/HIF-1 signal pathway in RA-FLS cells.

【 授权许可】

CC BY-NC