Beta-caryophyllene prevents the defects in trabecular bone caused by Vitamin D deficiency through pathways instated by increased expression of klotho

Bone & Joint Research

article

Wei Dong¹  Bradley C. Postlethwaite²  Patricia A. Wheller²  David Brand²  Yan Jiao¹  Wei Li³  Linda K. Myers²  Weikuan Gu¹ 

[1] Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center;Department of Medicine, University of Tennessee Health Science Center;Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

关键词: β-caryophyllene;    Vitamin D;    Bone loss;    Bone density;    Vitamin D deficiency;    trabecular bones;    vitamin D+;    serum;    femora;    bone mineral density (BMD);    tibial bone;    Cortical bone;    enzyme-linked immunosorbent assay;    fibroblast growth factor;   

DOI  :  10.1302/2046-3758.118.BJR-2021-0392.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsThis study investigated the effects of β-caryophyllene (BCP) on protecting bone from vitamin D deficiency in mice fed on a diet either lacking (D-) or containing (D+) vitamin D.MethodsA total of 40 female mice were assigned to four treatment groups (n = 10/group): D+ diet with propylene glycol control, D+ diet with BCP, D-deficient diet with control, and D-deficient diet with BCP. The D+ diet is a commercial basal diet, while the D-deficient diet contains 0.47% calcium, 0.3% phosphorus, and no vitamin D. All the mice were housed in conditions without ultraviolet light. Bone properties were evaluated by X-ray micro-CT. Serum levels of klotho were measured by enzyme-linked immunosorbent assay.ResultsUnder these conditions, the D-deficient diet enhanced the length of femur and tibia bones (p < 0.050), and increased bone volume (BV; p < 0.010) and trabecular bone volume fraction (BV/TV; p < 0.010) compared to D+ diet. With a diet containing BCP, the mice exhibited higher BV and bone mineral density (BMD; p < 0.050) than control group. The trabecular and cortical bone were also affected by vitamin D and BCP. In addition, inclusion of dietary BCP improved the serum concentrations of klotho (p < 0.050). In mice, klotho regulates the expression level of cannabinoid type 2 receptor (Cnr2) and fibroblast growth factor 23 (Fgf23) through CD300a. In humans, data suggest that klotho is connected to BMD. The expression of klotho is also associated with bone markers.ConclusionThese data indicate that BCP enhances the serum level of klotho, leading to improved bone properties and mineralization in an experimental mouse model.

【 授权许可】

CC BY-NC