Modelling osteoarthritis in mice via surgical destabilization of the medial meniscus with or without a stereomicroscope

Bone & Joint Research

article

Wencheng Hu¹  Junqing Lin¹  Jiabao Wei¹  Yunlong Yang²  Kai Fu¹  Tianhao Zhu¹  Hongyi Zhu¹  Xianyou Zheng¹ 

[1] Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital;Institute of Microsurgery on Extremities, Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital

关键词: Osteoarthritis;    DMM;    Microscopy;    Cartilage degeneration;    Joint pain;    Osteoarthritis (OA);    medial meniscus;    DMM: surgery;    OARSI;    Gait analysis;    destabilization of the medial meniscus (DMM) surgery;    sham surgery;    t-tests;    knee joint;   

DOI  :  10.1302/2046-3758.118.BJR-2021-0575.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsTo evaluate inducing osteoarthritis (OA) by surgical destabilization of the medial meniscus (DMM) in mice with and without a stereomicroscope.MethodsBased on sample size calculation, 70 male C57BL/6 mice were randomly assigned to three surgery groups: DMM aided by a stereomicroscope; DMM by naked eye; or sham surgery. The group information was blinded to researchers. Mice underwent static weightbearing, von Frey test, and gait analysis at two-week intervals from eight to 16 weeks after surgery. Histological grade of OA was determined with the Osteoarthritis Research Society International (OARSI) scoring system.ResultsSurgical DMM with or without stereomicroscope led to decrease in the mean of weightbearing percentages (-20.64% vs -21.44%, p = 0.792) and paw withdrawal response thresholds (-21.35% vs -24.65%, p = 0.327) of the hind limbs. However, the coefficient of variation (CV) of weight-bearing percentages and paw withdrawal response thresholds in naked-eye group were significantly greater than that in the microscope group (19.82% vs 6.94%, p < 0.001; 21.85% vs 9.86%, p < 0.001). The gait analysis showed a similar pattern. Cartilage degeneration was observed in both DMM-surgery groups, evidenced by increased OARSI scores (summed score: 11.23 vs 11.43, p = 0.842), but the microscope group showed less variation in OARSI score than the naked-eye group (CV: 21.03% vs 32.44%; p = 0.032).ConclusionAlthough surgical DMM aided by stereomicroscope is technically difficult, it produces a relatively more homogeneous OA model in terms of the discrete degree of pain behaviours and histopathological grading when compared with surgical DMM without stereomicroscope.

【 授权许可】

CC BY-NC