The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease

Bone & Joint Research

article

Mei Ma¹  Xiao Liang¹  Xi Wang¹  Lu Zhang¹  Shiqiang Cheng¹  Xiong Guo¹  Feng Zhang¹  Yan Wen¹ 

[1] School of Public Health, Health Science Center, Xi’an Jiaotong University

关键词: Kashin-Beck disease;    COMP;    Chondrocyte;    Apoptosis;    T-2 toxin;   

DOI  :  10.1302/2046-3758.99.BJR-2019-0247.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsKashin-Beck disease (KBD) is a kind of chronic osteochondropathy, thought to be caused by environmental risk factors such as T-2 toxin. However, the exact aetiology of KBD remains unclear. In this study, we explored the functional relevance and biological mechanism of cartilage oligosaccharide matrix protein (COMP) in the articular cartilage damage of KBD.MethodsThe articular cartilage specimens were collected from five KBD patients and five control subjects for cell culture. The messenger RNA (mRNA) and protein expression levels were detected by quantitative reverse transcription PCR (qRT-PCR) and western blot. The survival rate of C28/I2 chondrocyte cell line was detected by MTT assay after T-2 toxin intervention. The cell viability and mRNA expression levels of apoptosis related genes between COMP-overexpression groups and control groups were examined after cell transfection.ResultsThe mRNA and protein expression levels of COMP were significantly lower in KBD chondrocytes than control chondrocytes. After the T-2 toxin intervention, the COMP mRNA expression of C28/I2 chondrocyte reduced and the protein level of COMP in three intervention groups was significantly lower than in the control group. MTT assay showed that the survival rate of COMP overexpression KBD chondrocytes were notably higher than in the blank control group. The mRNA expression levels of Survivin, SOX9, Caspase-3, and type II collagen were also significantly different among COMP overexpression, negative control, and blank control groups.ConclusionOur study results confirmed the functional relevance of COMP with KBD. COMP may play an important role in the excessive chondrocytes apoptosis of KBD patients.

【 授权许可】

CC BY-NC