Cordycepin inhibits LPS-induced inflammatory and matrix degradation in the intervertebral disc

PeerJ

article

Yan Li¹  Kang Li¹  Lu Mao²  Xiuguo Han¹  Kai Zhang¹  Changqing Zhao¹  Jie Zhao¹ 

[1] Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedics, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University School of Medicine;Spine Center, Zhongda Hospital, School of Medicine, Southeast University

关键词: Cordycepin;    Intervertebral disc;    Nucleus pulposus cells;    Degeneration;    Inflammation;   

DOI  :  10.7717/peerj.1992

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Cordycepin is a component of the extract obtained from Cordyceps militaris and has many biological activities, including anti-cancer, anti-metastatic and anti-inflammatory effects. Intervertebral disc degeneration (IDD) is a degenerative disease that is closely related to the inflammation of nucleus pulposus (NP) cells. The effect of cordycepin on NP cells in relation to inflammation and degeneration has not yet been studied. In our study, we used a rat NP cell culture and an intervertebral disc (IVD) organ culture model to examine the inhibitory effects of cordycepin on lipopolysaccharide (LPS)-induced gene expression and the production of matrix degradation enzymes (MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5) and oxidative stress-associated factors (nitric oxide and PGE2). We found a protective effect of cordycepin on NP cells and IVDs against LPS-induced matrix degradation and macrophage infiltration. In addition, western blot and luciferase assay results demonstrated that pretreatment with cordycepin significantly suppressed the LPS-induced activation of the NF-κB pathway. Taken together, the results of our research suggest that cordycepin could exert anti-inflammatory and anti-degenerative effects on NP cells and IVDs by inhibiting the activation of the NF-κB pathway. Therefore, cordycepin may be a potential treatment for IDD in the future.

【 授权许可】

CC BY