European Spine Journal | |
Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential | |
Thomas Steffen2  Peter Roughley1  Janet Moir2  Lisbet Haglund2  Bernice Jim2  | |
[1] Shriners Hospital for Children, McGill University, 1529 Cedar Avenue, Montreal, QC H3G 1AG Canada;Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, Montreal, QC H3A 1A1 Canada | |
关键词: Intervertebral disc; Degeneration; Repair; Organ culture; | |
DOI : 10.1007/s00586-011-1721-x | |
学科分类:骨科学 | |
来源: Springer | |
【 摘 要 】
The present work describes a novel bovine disc organ culture system with long-term maintenance of cell viability, in which degenerative changes can be induced as a prelude to studying repair. Discs were isolated with three different techniques: without endplates (NEP), with bony endplates (BEP) and with intact cartilage endplates (CEP). Swelling, deformation, and cell viability were evaluated in unloaded cultures. Degeneration was induced by a single trypsin injection into the center of the disc and the effect on cell viability and matrix degradation was followed. Trypsin-treated discs were exposed to TGFβ to evaluate the potential to study repair in this system. NEP isolated discs showed >75% maintained cell viability for up to 10 days but were severely deformed, BEP discs on the other hand maintained morphology but failed to retain cell viability having only 27% viable cells after 10 days. In CEP discs, both cell viability and morphology were maintained for at least 4 weeks where >75% of the cells were still viable. To mimic proteoglycan loss during disc degeneration, a single trypsin injection was administered to the center of the disc. This resulted in 60% loss of aggrecan, after 7 days, without affecting cell viability. When TGFβ was injected to validate that the system can be used to study a repair response following injection of a bio-active substance, proteoglycan synthesis nearly doubled compared to baseline synthesis. Trypsin-treated bovine CEP discs therefore provide a model system for studying repair of the degenerate disc, as morphology, cell viability and responsiveness to bio-active substances were maintained.
【 授权许可】
Unknown
【 预 览 】
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RO201911300237890ZK.pdf | 1293KB | download |