Transcriptomic analysis reveals distinct resistant response by physcion and chrysophanol against cucumber powdery mildew

PeerJ

article

Yanping Li¹  Shilin Tian²  Xiaojun Yang¹  Xin Wang¹  Yuhai Guo¹  Hanwen Ni¹ 

[1] College of Agronomy and Biotechnology, China Agricultural University;Novogene Bioinformatics Institute;Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University

关键词: Cucumber;    Transcriptomic;    Gene expression;    RNA-seq;   

DOI  :  10.7717/peerj.1991

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Physcion and chrysophanol induce defense responses against powdery mildew in cucumbers. The combination of these two compounds has synergistic interaction against the disease. We performed RNA-seq on cucumber leaf samples treated with physcion and chrysophanol alone and with their combination. We generated 17.6 Gb of high-quality sequencing data (∼2 Gb per sample) and catalogued the expressions profiles of 12,293 annotated cucumber genes in each sample. We identified numerous differentially expressed genes that exhibited distinct expression patterns among the three treatments. The gene expression patterns of the Chr and Phy treatments were more similar to each other than to the Phy × Chr treatment. The Phy × Chr treatment induced the highest number of differentially expressed genes. This dramatic transcriptional change after Phy × Chr treatment leaves reflects that physcion combined with chrysophanol treatment was most closely associated with induction of disease resistance. The analysis showed that the combination treatment caused expression changes of numerous defense-related genes. These genes have known or potential roles in structural, chemical and signaling defense responses and were enriched in functional gene categories potentially responsible for cucumber resistance. These results clearly demonstrated that disease resistance in cucumber leaves was significantly influenced by the combined physcion and chrysophanol treatment. Thus, physcion and chrysophanol are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the defense response.

【 授权许可】

CC BY