The oral microbiome of patients with axial spondyloarthritis compared to healthy individuals

PeerJ

article

Jordan E. Bisanz¹  Praema Suppiah³  W. Murray Thomson³  Trudy Milne³  Nigel Yeoh⁵  Anita Nolan⁵  Grace Ettinger¹  Gregor Reid¹  Gregory B. Gloor²  Jeremy P. Burton¹  Mary P. Cullinan³  Simon M. Stebbings⁵ 

[1] Department of Microbiology and Immunology, Western University;Canadian Centre for Human Microbiome and Probiotic Research, Lawson Health Research Institute;School of Dentistry, University of Otago;Sir John Walsh Research Institute, University of Otago;Dunedin School of Medicine, University of Otago;Oral Health;Division of Urology, Department of Surgery, University of Western Ontario;Department of Biochemistry, University of Western Ontario

关键词: Periodontal disease;    Microbiome;    Oral cavity;    Axial spondyloarthritis;   

DOI  :  10.7717/peerj.2095

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background. A loss of mucosal tolerance to the resident microbiome has been postulated in the aetiopathogenesis of spondyloarthritis, thus the purpose of these studies was to investigate microbial communities that colonise the oral cavity of patients with axial spondyloarthritis (AxSpA) and to compare these with microbial profiles of a matched healthy population.Methods. Thirty-nine participants, 17 patients with AxSpA and 22 age and gender-matched disease-free controls were recruited to the study. For patients with AxSpA, disease activity was assessed using the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). All participants underwent a detailed dental examination to assess oral health, including the presence of periodontal disease assessed using probing pocket depth (PPD). Plaque samples were obtained and their bacterial populations were profiled using Ion Torrent sequencing of the V6 region of the 16S rRNA gene.Results.Patients with AxSpA had active disease (BASDAI 4.1 ± 2.1 [mean ± SD]), and a significantly greater prevalence of periodontitis (PPD ≥ 4 mm at ≥4 sites) than controls. Bacterial communities did not differ between the two groups with multiple metrics of α and β diversity considered. Analysis of operational taxonomic units (OTUs) and higher levels of taxonomic assignment did not provide strong evidence of any single taxa associated with AxSpA in the subgingival plaque.Discussion. Although 16S rRNA gene sequencing did not identify specific bacterial profiles associated with AxSpA, there remains the potential for the microbiota to exert functional and metabolic influences in the oral cavity which could be involved in the pathogenesis of AxSpA.

【 授权许可】

CC BY