Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development

PeerJ

article

Jeanne A. Robert¹  Tiffany Bonnett¹  Caitlin Pitt¹  Luke J. Spooner¹  Jordie Fraser¹  Macaire M.S. Yuen²  Christopher I. Keeling²  Jörg Bohlmann²  Dezene P.W. Huber¹ 

[1] Department of Ecosystem Science and Management, University of Northern British Columbia, Prince George;Michael Smith Laboratories, University of British Columbia;Department of Biological Sciences, Simon Fraser University

关键词: Cold tolerance;    Mountain pine beetle;    Insect overwintering;    Glycerol;    Physiological stress;    Lodgepole pine;    Heat shock proteins;    Anti-freeze;    Cytochromes P450;   

DOI  :  10.7717/peerj.2109

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Cold-induced mortality has historically been a key aspect of mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. We used RNA-seq analysis to monitor gene expression patterns of mountain pine beetle larvae at four time points during their overwintering period—early-autumn, late-autumn, early-spring, and late-spring. Changing transcript profiles over the winter indicates a multipronged physiological response from larvae that is broadly characterized by gene transcripts involved in insect immune responses and detoxification during the autumn. In the spring, although transcripts associated with developmental process are present, there was no particular biological process dominating the transcriptome.

【 授权许可】

CC BY