Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae

PeerJ

article

Jordie D. Fraser¹  Tiffany R. Bonnett¹  Christopher I. Keeling²  Dezene P.W. Huber¹ 

[1] Ecosystem Science and Management Program, University of Northern British Columbia, Prince George;Department of Biological Sciences, Simon Fraser University;Michael Smith Laboratories, University of British Columbia

关键词: Bark beetle;    Mountain pine beetle;    Overwintering;    Glycerol;    Seasonal;    Cyroprotectant;    Freeze intolerant;    Dendroctonus ponderosae;    Coleoptera;    Curculionidae;   

DOI  :  10.7717/peerj.3284

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Winter mortality is a major factor regulating population size of the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). Glycerol is the major cryoprotectant in this freeze intolerant insect. We report findings from a gene expression study on an overwintering mountain pine beetle population over the course of 35 weeks. mRNA transcript levels suggest glycerol production in the mountain pine beetle occurs through glycogenolytic, gluconeogenic and potentially glyceroneogenic pathways, but not from metabolism of lipids. A two-week lag period between fall glycogen phosphorylase transcript and phosphoenolpyruvate carboxykinase transcript up-regulation suggests that gluconeogenesis serves as a secondary glycerol-production process, subsequent to exhaustion of the primary glycogenolytic source. These results provide a first look at the details of seasonal gene expression related to the production of glycerol in the mountain pine beetle.

【 授权许可】

CC BY