XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

PeerJ

article

Nicholas C. Thomas¹  Benjamin Schwessinger¹  Furong Liu¹  Huamin Chen¹  Tong Wei¹  Yen P. Nguyen¹  Isaac W.F. Shaker¹  Pamela C. Ronald¹ 

[1] Department of Plant Pathology and the Genome Center, University of California;Joint BioEnergy Institute;Research School of Biology, Australian National University;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences

关键词: XA21;    Xanthomonas;    EFR;    Rice;    RNAseq;   

DOI  :  10.7717/peerj.2446

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

【 授权许可】

CC BY