The rice XA21 ectodomain fused to the Arabidopsis EFR cytoplasmic domain confers resistance to Xanthomonas oryzae pv. oryzae

PeerJ

article

Nicholas C. Thomas¹  Nir Oksenberg¹  Furong Liu¹  Daniel Caddell¹  Alina Nalyvayko¹  Yen Nguyen¹  Benjamin Schwessinger¹  Pamela C. Ronald¹ 

[1] Department of Plant Pathology and the Genome Center, University of California

关键词: Rice;    XA21;    EFR;    Disease resistance;    Xanthomonas;    Immune receptor;    Chimeric receptor;   

DOI  :  10.7717/peerj.4456

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Rice (Oryza sativa) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. oryzae (Xoo) infection. We previously demonstrated that expressing a chimeric protein containing the ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and the XA21 endodomain (EFR:XA21) in rice does not confer robust resistance to Xoo. To test if the XA21 ectodomain is required for Xoo resistance, we produced transgenic rice lines expressing a chimeric protein consisting of the XA21 ectodomain and EFR endodomain (XA21:EFR) and inoculated these lines with Xoo. We also tested if the XA21:EFR rice plants respond to a synthetic sulfated 21 amino acid derivative (RaxX21-sY) of the activator of XA21-mediated immunity, RaxX. We found that five independently transformed XA21:EFR rice lines displayed resistance to Xoo as measured by lesion length analysis, and showed that five lines share characteristic markers of the XA21 defense response (generation of reactive oxygen species and defense response gene expression) after treatment with RaxX21-sY. Our results indicate that expression of the XA21:EFR chimeric receptor in rice confers resistance to Xoo. These results suggest that the endodomain of the EFR and XA21 immune receptors are interchangeable and the XA21 ectodomain is the key determinant conferring robust resistance to Xoo.

【 授权许可】

CC BY