Salinity modulates thermotolerance, energy metabolism and stress response in amphipods Gammarus lacustris

PeerJ

article

Kseniya P. Vereshchagina¹  Yulia A. Lubyaga¹  Zhanna Shatilina¹  Daria Bedulina¹  Anton Gurkov¹  Denis V. Axenov-Gribanov¹  Boris Baduev¹  Elizaveta S. Kondrateva¹  Mikhail Gubanov³  Egor Zadereev³  Inna Sokolova⁵  Maxim Timofeyev¹ 

[1] Institute of Biology, Irkutsk State University;Baikal Research Centre;Institute of Biophysics SB RAS;Siberian Federal University;Institute for Biological Sciences, University of Rostock

关键词: Thermal tolerance;    Salinity;    Gammarus lacustris;    Adaptation;    Amphipoda;   

DOI  :  10.7717/peerj.2657

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was observed, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress.

【 授权许可】

CC BY