Metabolic phenotype of clinical and environmental Mycobacterium avium subsp. hominissuis isolates

PeerJ

article

Andrea Sanchini¹  Flavia Dematheis²  Torsten Semmler³  Astrid Lewin¹ 

[1] Division 16, Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute;Institute of Microbiology and Epizootics, Free University Berlin;NG 1 Microbial Genomics, Robert Koch Institute

关键词: Mycobacterium avium;    Biolog;    OmniLog®;    Metabolism;    Phenotype microarray;   

DOI  :  10.7717/peerj.2833

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundMycobacterium avium subsp. hominissuis (MAH) is an emerging opportunistic human pathogen. It can cause pulmonary infections, lymphadenitis and disseminated infections in immuno-compromised patients. In addition, MAH is widespread in the environment, since it has been isolated from water, soil or dust. In recent years, knowledge on MAH at the molecular level has increased substantially. In contrast, knowledge of the MAH metabolic phenotypes remains limited.MethodsIn this study, for the first time we analyzed the metabolic substrate utilization of ten MAH isolates, five from a clinical source and five from an environmental source. We used BIOLOG Phenotype MicroarrayTM technology for the analysis. This technology permits the rapid and global analysis of metabolic phenotypes.ResultsThe ten MAH isolates tested showed different metabolic patterns pointing to high intra-species diversity. Our MAH isolates preferred to use fatty acids such as Tween, caproic, butyric and propionic acid as a carbon source, and L-cysteine as a nitrogen source. Environmental MAH isolates resulted in being more metabolically active than clinical isolates, since the former metabolized more strongly butyric acid (p = 0.0209) and propionic acid (p = 0.00307).DiscussionOur study provides new insight into the metabolism of MAH. Understanding how bacteria utilize substrates during infection might help the developing of strategies to fight such infections.

【 授权许可】

CC BY