Single exponential decay waveform; a synergistic combination of electroporation and electrolysis (E2) for tissue ablation

PeerJ

article

Nina Klein¹  Enric Guenther¹  Paul Mikus¹  Michael K. Stehling¹  Boris Rubinsky¹ 

[1] Inter Science GmbH;Prostata Center, Institut fur Bildgebende Diagnostik;Department of Mechanical Engineering, University of California

关键词: Tissue ablation;    Synergy electroporation and electrolysis;    Liver;    Electrolytic ablation;    Reversible electroporation;    Irreversible electroporation;    Electrolysis;   

DOI  :  10.7717/peerj.3190

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundElectrolytic ablation and electroporation based ablation are minimally invasive, non-thermal surgical technologies that employ electrical currents and electric fields to ablate undesirable cells in a volume of tissue. In this study, we explore the attributes of a new tissue ablation technology that simultaneously delivers a synergistic combination of electroporation and electrolysis (E2).MethodA new device that delivers a controlled dose of electroporation field and electrolysis currents in the form of a single exponential decay waveform (EDW) was applied to the pig liver, and the effect of various parameters on the extent of tissue ablation was examined with histology.ResultsHistological analysis shows that E2 delivered as EDW can produce tissue ablation in volumes of clinical significance, using electrical and temporal parameters which, if used in electroporation or electrolysis separately, cannot ablate the tissue.DiscussionThe E2 combination has advantages over the three basic technologies of non-thermal ablation: electrolytic ablation, electrochemical ablation (reversible electroporation with injection of drugs) and irreversible electroporation. E2 ablates clinically relevant volumes of tissue in a shorter period of time than electrolysis and electroporation, without the need to inject drugs as in reversible electroporation or use paralyzing anesthesia as in irreversible electroporation.

【 授权许可】

CC BY