Modeling the cis -regulatory modules of genes expressed in developmental stages of Drosophila melanogaster

PeerJ

article

Yosvany López¹  Alexis Vandenbon³  Akinao Nose⁴  Kenta Nakai¹ 

[1] Human Genome Center, The Institute of Medical Science, The University of Tokyo;Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo;Immunology Frontier Research Center, Osaka University;Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, The University of Tokyo

关键词: Promoter architecture;    Co-expression;    Genetic algorithm;    Transcription factor binding sites;    Developmental stage;    Genome-wide analysis;   

DOI  :  10.7717/peerj.3389

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Because transcription is the first step in the regulation of gene expression, understanding how transcription factors bind to their DNA binding motifs has become absolutely necessary. It has been shown that the promoters of genes with similar expression profiles share common structural patterns. This paper presents an extensive study of the regulatory regions of genes expressed in 24 developmental stages of Drosophila melanogaster. It proposes the use of a combination of structural features, such as positioning of individual motifs relative to the transcription start site, orientation, pairwise distance between motifs, and presence of motifs anywhere in the promoter for predicting gene expression from structural features of promoter sequences. RNA-sequencing data was utilized to create and validate the 24 models. When genes with high-scoring promoters were compared to those identified by RNA-seq samples, 19 (79.2%) statistically significant models, a number that exceeds previous studies, were obtained. Each model yielded a set of highly informative features, which were used to search for genes with similar biological functions.

【 授权许可】

CC BY