Calcitonin receptor expression in medullary thyroid carcinoma

PeerJ

article

Virginia Cappagli¹  Catarina Soares Potes³  Luciana Bueno Ferreira¹  Catarina Tavares¹  Catarina Eloy¹  Rossella Elisei²  Manuel Sobrinho-Simões¹  Peter J. Wookey⁹  Paula Soares¹ 

[1] Cancer Signaling and Metabolism Group, Institute of Molecular Pathology and Immunology of the University of Porto;Department of Clinical and Experimental Medicine, Endocrine Unit, University of Pisa;Instituto de Investigação e Inovação em Saúde, Universidade do Porto;Institute for Molecular and Cell Biology ,(IBMC), University of Porto;Department of Biomedicine – Experimental Biology Unit, Faculty of Medicine, University of Porto;Medical Faculty, University of Porto;Department of Pathology, Hospital de S. João;Department of Pathology, Medical Faculty, University of Porto;Department of Medicine at Austin Health, University of Melbourne

关键词: Calcitonin receptor;    Calcitonin;    C-cells;    Medullary thyroid carcinoma;   

DOI  :  10.7717/peerj.3778

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Calcitonin expression is a well-established marker for medullary thyroid carcinoma (MTC); yet the role of calcitonin receptor (CTR), its seven-transmembrane G-protein coupled receptor, remains to be established in C-cells derived thyroid tumors. The aim of this work was to investigate CTR expression in MTC and to correlate such expression with clinicopathological features in order to evaluate its possible role as a prognostic indicator of disease aggressiveness and outcome. Methods Calcitonin receptor expression was analyzed in a series of 75 MTCs by immunohistochemistry, and by qPCR mRNA quantification in specimens from four patients. Statistical tests were used to evaluate the correlation between CTR expression and the clinicopathological and molecular characteristics of patients and tumors. Results Calcitonin receptor expression was detected in 62 out of 75 samples (82.7%), whereas 13 of the 75 samples (17.3%) were completely negative. CTR expression was significantly associated with expression of cytoplasmatic phosphatase and tensin homologue deleted on chromosome 10 and osteopontin, as well as with wild type RET/RAS genes and absence of tumor stroma, suggesting that CTR expression do not associate with clinicopathological signs of worse prognosis. Discussion Calcitonin receptor expression appears to be associated in MTC with more differentiated status of the neoplastic cells.

【 授权许可】

CC BY