A novel calcitonin receptor gene in human osteoclasts from normal bone marrow

FEBS Letters

Nishikawa, Takuji²  Koshihara, Yasuko¹  Yamamoto, Seizo²  Ishikawa, Hirokazu³ 

[1] Department of Nutrition, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 1730015, Japan;Department of Orthopaedic Surgery, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan;Pharmacology and Molecular Biology Laboratories Sankyo, Tokyo, Japan

关键词: Calcitonin receptor;    Human;    Osteoclast;    MCF-7;    Calcitonin;    Gene;    CT;    calcitonin;    CTR;    calcitonin receptor;    hCT;    recombinant human calcitonin;    MNC;    multinucleated cell;    PCR;    polymerase chain reaction;    MEM;    minimum essential medium;   

DOI  :  10.1016/S0014-5793(99)01176-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The calcitonin receptor (CTR) gene in human osteoclasts formed in a human bone marrow cell culture system was examined by reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR results indicated that the 5′-untranslated region (5′UTR) was different between CTR mRNAs in human osteoclasts and in a mammary tumor cell line, MCF-7 cells. We isolated the 5′UTR of the CTR gene from human osteoclasts, whose sequence had only 28.6% identity with that of other CTR genes reported until now. In a radioligand binding assay, COS-1 cells transfected with the osteoclast CTR gene bound to [¹²⁵I]human CT (hCT). These results provided evidence that the CTR gene cloned from human osteoclasts was expressed functionally and its coding protein was identical to MCF-7 cell CTR.

【 授权许可】

Unknown