The complete chloroplast genome sequence of strawberry ( Fragaria × ananassa Duch.) and comparison with related species of Rosaceae

PeerJ

article

Hui Cheng¹  Jinfeng Li²  Hong Zhang¹  Binhua Cai¹  Zhihong Gao¹  Yushan Qiao¹  Lin Mi² 

[1]Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University

[2]Laboratory of Fruit Tree, Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province

关键词: Fragaria × ananassa Duch.;    Benihoppe;    Chloroplast genome;    Comparative analysis;    Chloroplast DNA markers;   

DOI  :  10.7717/peerj.3919

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Compared with other members of the family Rosaceae, the chloroplast genomes of Fragaria species exhibit low variation, and this situation has limited phylogenetic analyses; thus, complete chloroplast genome sequencing of Fragaria species is needed. In this study, we sequenced the complete chloroplast genome of F. × ananassa ‘Benihoppe’ using the Illumina HiSeq 2500-PE150 platform and then performed a combination of de novo assembly and reference-guided mapping of contigs to generate complete chloroplast genome sequences. The chloroplast genome exhibits a typical quadripartite structure with a pair of inverted repeats (IRs, 25,936 bp) separated by large (LSC, 85,531 bp) and small (SSC, 18,146 bp) single-copy (SC) regions. The length of the F. × ananassa ‘Benihoppe’ chloroplast genome is 155,549 bp, representing the smallest Fragaria chloroplast genome observed to date. The genome encodes 112 unique genes, comprising 78 protein-coding genes, 30 tRNA genes and four rRNA genes. Comparative analysis of the overall nucleotide sequence identity among ten complete chloroplast genomes confirmed that for both coding and non-coding regions in Rosaceae, SC regions exhibit higher sequence variation than IRs. The Ka/Ks ratio of most genes was less than 1, suggesting that most genes are under purifying selection. Moreover, the mVISTA results also showed a high degree of conservation in genome structure, gene order and gene content in Fragaria, particularly among three octoploid strawberries which were F. × ananassa ‘Benihoppe’, F. chiloensis (GP33) and F. virginiana (O477). However, when the sequences of the coding and non-coding regions of F. × ananassa ‘Benihoppe’ were compared in detail with those of F. chiloensis (GP33) and F. virginiana (O477), a number of SNPs and InDels were revealed by MEGA 7. Six non-coding regions (trnK-matK, trnS-trnG, atpF-atpH, trnC-petN, trnT-psbD and trnP-psaJ) with a percentage of variable sites greater than 1% and no less than five parsimony-informative sites were identified and may be useful for phylogenetic analysis of the genus Fragaria.

【 授权许可】

CC BY