A novel animal model for neuroinflammation and white matter degeneration

PeerJ

article

Baohu Ji¹  Kerin Higa¹  Virawudh Soontornniyomkij¹  Atsushi Miyanohara²  Xianjin Zhou¹ 

[1] Department of Psychiatry, University of California;Department of Anesthesiology, University of California;Research Service, VA San Diego Healthcare System

关键词: shRNA;    Drd1;    Microglial activation;    Innate immunity;    Neuroinflammation;    White matter degeneration;   

DOI  :  10.7717/peerj.3905

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Small interference RNA has been widely used to suppress gene expression. Three different short hairpin RNAs (shRNAs) against dopamine D1 receptor (Drd1), driven by mouse U6 promoter in self-complementary AAV8 vector (scAAV8), were used to silence mouse striatal Drd1 expression. Transduction of mouse striatum with all three scAAV8-D1shRNA viruses, but not the control scAAV8 virus, causes extensive neuroinflammation, demyelination, and axon degeneration. RNA interference is known to be coupled to the innate immune system as a host cell defense against virus infection. Activation of the innate immune system may play a causal role in the development of neuroinflammation and white matter degeneration, providing a novel animal model for multiple sclerosis (MS) and other neuroinflammatory diseases.

【 授权许可】

CC BY