The sensitivity of the yeast, Saccharomyces cerevisiae , to acetic acid is influenced by DOM34 and RPL36A

PeerJ

article

Bahram Samanfar¹  Kristina Shostak¹  Houman Moteshareie¹  Maryam Hajikarimlou¹  Sarah Shaikho³  Katayoun Omidi¹  Mohsen Hooshyar¹  Daniel Burnside¹  Imelda Galván Márquez¹  Tom Kazmirchuk¹  Thet Naing³  Paula Ludovico⁵  Anna York-Lyon¹  Kama Szereszewski¹  Cindy Leung¹  Jennifer Yixin Jin¹  Rami Megarbane¹  Myron L. Smith¹  Mohan Babu⁷  Martin Holcik³  Ashkan Golshani¹ 

[1] Department of Biology and Ottawa Institute of Systems Biology, Carleton University;Agriculture and Ari-Food Canada, Ottawa Research and Development Centre;Children’s Hospital of Eastern Ontario Research Institute, Department of Pediatrics , University of Ottawa;Ottawa Hospital Research Institute, Center for Cancer Therapeutics;Life and Health Sciences Research Institute ,(ICVS), School of Health Sciences, University of Minho;Department of Chemistry, Carleton University;Department of Biochemistry, Research and Innovation Centre, University of Regina;Department of Health Sciences, Carleton University

关键词: Yeast;    Gene deletion;    HSP;    Protein expression;    Saccharomyces cerevisiae;    Acetic acid;    Heat shock;    DOM34 and RPL36A;    DOM34;    RPL36A;   

DOI  :  10.7717/peerj.4037

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The presence of acetic acid during industrial alcohol fermentation reduces the yield of fermentation by imposing additional stress on the yeast cells. The biology of cellular responses to stress has been a subject of vigorous investigations. Although much has been learned, details of some of these responses remain poorly understood. Members of heat shock chaperone HSP proteins have been linked to acetic acid and heat shock stress responses in yeast. Both acetic acid and heat shock have been identified to trigger different cellular responses including reduction of global protein synthesis and induction of programmed cell death. Yeast HSC82 and HSP82 code for two important heat shock proteins that together account for 1–2% of total cellular proteins. Both proteins have been linked to responses to acetic acid and heat shock. In contrast to the overall rate of protein synthesis which is reduced, the expression of HSC82 and HSP82 is induced in response to acetic acid stress. In the current study we identified two yeast genes DOM34 and RPL36A that are linked to acetic acid and heat shock sensitivity. We investigated the influence of these genes on the expression of HSP proteins. Our observations suggest that Dom34 and RPL36A influence translation in a CAP-independent manner.

【 授权许可】

CC BY