Protective effect of Rhus coriaria fruit extracts against hydrogen peroxide-induced oxidative stress in muscle progenitors and zebrafish embryos

PeerJ

article

Fadia Najjar¹  Francine Rizk¹  Gilles Carnac²  Rim Nassar¹  Sara Jabak¹  Anatoly Petrovich Sobolev³  Yara Bou Saada⁴  Marwan El Sabban⁵  Aline Hamade¹ 

[1] Departments of Biology, Chemistry and Biochemistry, Laboratoire d’Innovation Thérapeutique, Faculty of Sciences, Lebanese University;PhyMedExp, University of Montpellier;Istituto di Metodologie Chimiche, CNR, Laboratorio di Risonanza Magnetica Nucleare “Annalaura Segre”;UMR 8256, CNRS, Université Pierre et Marie Curie;Department of Anatomy, Cell Biology and Physiological Sciences, American University of Beirut

关键词: Sumac Rhus coriaria extract;    Myoblast (LHCN-M2);    Aging;    NMR;    Antioxidants;    Zebrafish embryos;   

DOI  :  10.7717/peerj.4144

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background and PurposeOxidative stress is involved in normal and pathological functioning of skeletal muscle. Protection of myoblasts from oxidative stress may improve muscle contraction and delay aging. Here we studied the effect of R. coriaria sumac fruit extract on human myoblasts and zebrafish embryos in conditions of hydrogen peroxide-induced oxidative stress.Study Design and MethodsCrude ethanolic 70% extract (CE) and its fractions was obtained from sumac fruits. The composition of sumac ethyl acetate EtOAc fraction was studied by 1H NMR. The viability of human myoblasts treated with CE and the EtOAc fraction was determined by trypan blue exclusion test. Oxidative stress, cell cycle and adhesion were analyzed by flow cytometry and microscopy. Gene expression was analyzed by qPCR.ResultsThe EtOAc fraction (IC50 2.57 µg/mL) had the highest antioxidant activity and exhibited the best protective effect against hydrogen peroxide-induced oxidative stress. It also restored cell adhesion. This effect was mediated by superoxide dismutase 2 and catalase. Pre-treatment of zebrafish embryos with low concentrations of the EtOAc fraction protected them from hydrogen peroxide-induced death in vivo. 1H NMR analysis revealed the presence of gallic acid in this fraction.ConclusionRhus coriaria extracts inhibited or slowed down the progress of skeletal muscle atrophy by decreasing oxidative stress via superoxide dismutase 2 and catalase-dependent mechanisms.

【 授权许可】

CC BY