Impact of hypoxia stress on the physiological responses of sea cucumber Apostichopus japonicus : respiration, digestion, immunity and oxidative damage

PeerJ

article

Da Huo¹  Lina Sun¹  Xiaoshang Ru¹  Libin Zhang¹  Chenggang Lin¹  Shilin Liu¹  Xiaoke Xin¹  Hongsheng Yang¹ 

[1] CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology;University of Chinese Academy of Sciences

关键词: Echinoderm;    Anoxia;    Metabolism;    Aquatic environment;    Dissolved oxygen;    Physiological behavior;   

DOI  :  10.7717/peerj.4651

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Hypoxia is one of the most frequently occurring stressors confronted by industrial cultures of sea cucumber and can cause large economic losses and resource degradation. However, its responsive mechanisms are still lacking. In this paper, the physiological responses of Apostichopus japonicus to oxygen deficiency was illustrated, including induced oxidative response and immune defense and changed digestive enzymes activities. Significantly increased activities of alpha-amylase (AMS), acid phosphatase (ACP), lactate dehydrogenase, catalase, peroxidase, succinate dehydrogenase and higher content of malondialdehyde, and decreased activities of lipase and trypsin (TRY) were observed after hypoxia exposure (dissolved oxygen [DO] 2 mg/L). Expressions of key genes showed that AMS, peptidase, ACP, alkaline phosphatase, lysozyme, heat shock protein 70 and glutathione peroxidase were increased and TRY was decreased under hypoxia. With the decline of the DO level, the decreased tendency of oxygen consumption rates was different in varied weight groups. Moreover, respiratory trees were observed degraded under long-term hypoxia stress, thus leading a negative effect of respiration. These results could help to develop a better understanding of the responsive mechanism of sea cucumber under hypoxia stress and provide a theoretical basis for the prevention of hypoxia risk.

【 授权许可】

CC BY