Biofilms and antibiotic susceptibility of multidrug-resistant bacteria from wild animals

PeerJ

article

Carla Dias¹  Anabela Borges¹  Diana Oliveira¹  Antonio Martinez-Murcia⁵  Maria José Saavedra²  Manuel Simões¹ 

[1] LEPABE-Department of Chemical Engineering, Faculty of Engineering—University of Porto;CITAB, Centre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Tras-os-Montes e Alto Douro;CECAV, Veterinary and Animal Science Research Center, Universidade de Tras-os-Montes e Alto Douro;CIQUP, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto;Area of Microbiology, EPSO, University Miguel Hernández

关键词: Biofilms;    Ciprofloxacin;    Imipenem;    Wild animals;    Multidrug-resistance;    β-lactamases;   

DOI  :  10.7717/peerj.4974

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe “One Health” concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance.MethodsThe purpose of this work was to assess the antibiotic susceptibility of four bacteria (Acinetobacter spp., Klebsiella pneumoniae, Pseudomonas fluorescens and Shewanella putrefaciens) isolated from wild animals and their ability to form biofilms. The effect of two antibiotics, imipenem (IPM) and ciprofloxacin (CIP), on biofilm removal was also assessed. Screening of resistance genetic determinants was performed by PCR. Biofilm tests were performed by a modified microtiter plate method. Bacterial surface hydrophobicity was determined by sessile drop contact angles.ResultsThe susceptibility profile classified the bacteria as multidrug-resistant. Three genes coding for β-lactamases were detected in K. pneumoniae (TEM, SHV, OXA-aer) and one in P. fluorescens (OXA-aer). K. pneumoniae was the microorganism that carried more β-lactamase genes and it was the most proficient biofilm producer, while P. fluorescens demonstrated the highest adhesion ability. Antibiotics at their MIC, 5 × MIC and 10 × MIC were ineffective in total biofilm removal. The highest biomass reductions were found with IPM (54% at 10 × MIC) against K. pneumoniae biofilms and with CIP (40% at 10 × MIC) against P. fluorescens biofilms.DiscussionThe results highlight wildlife as important host reservoirs and vectors for the spread of multidrug-resistant bacteria and genetic determinants of resistance. The ability of these bacteria to form biofilms should increase their persistence.

【 授权许可】

CC BY