Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

PeerJ

article

Melissa E. Carew¹  Rhys A. Coleman²  Ary A. Hoffmann¹ 

[1] School of BioSciences, The University of Melbourne;Applied Research

关键词: DNA barcoding;    Aquatic;    Metagenomics;    Insect;    Macroinvertebrates;    Bioassessment;    Freshwater;   

DOI  :  10.7717/peerj.4980

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundHigh throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring. As metabarcoding becomes more widely applied, new reference DNA barcodes linked to individual specimens identified by taxonomists are needed. This can be achieved through using DNA extraction methods that are not only suitable for metabarcoding but also for building reference DNA barcode libraries.MethodsIn this study, we test the suitability of a rapid non-destructive DNA extraction method for metabarcoding of freshwater invertebrate samples.ResultsThis method resulted in detection of taxa from many taxonomic groups, comparable to results obtained with two other tissue-based extraction methods. Most taxa could also be successfully used for subsequent individual-based DNA barcoding and taxonomic identification. The method was successfully applied to field-collected invertebrate samples stored for taxonomic studies in 70% ethanol at room temperature, a commonly used storage method for freshwater samples.DiscussionWith further refinement and testing, non-destructive extraction has the potential to rapidly characterise species biodiversity in invertebrate samples, while preserving specimens for taxonomic investigation.

【 授权许可】

CC BY