Genetic analysis of water loss of excised leaves associated with drought tolerance in wheat

PeerJ

article

Ilona Mieczysława Czyczyło-Mysza¹  Izabela Marcińska¹  Edyta Skrzypek¹  Jan Bocianowski²  Kinga Dziurka¹  Dragana Rančić³  Radenko Radošević³  Sofija Pekić-Quarrie³  Dejan Dodig⁴  Stephen Alexander Quarrie⁵ 

[1] Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology;Department of Mathematical and Statistical Methods, Poznań University of Life Sciences;Faculty of Agriculture, University of Belgrade;Maize Research Institute Zemun Polje;Newcastle University Business School;Faculty of Biology, Belgrade University

关键词: Morphology;    Leaf size;    QTL analysis;    Stomata;    Anatomy;    Yield;    Excised-leaf water loss (ELWL);    Wheat (Triticum aestivum L.);   

DOI  :  10.7717/peerj.5063

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundWheat is widely affected by drought. Low excised-leaf water loss (ELWL) has frequently been associated with improved grain yield under drought. This study dissected the genetic control of ELWL in wheat, associated physiological, morphological and anatomical leaf traits, and compared these with yield QTLs.MethodsNinety-four hexaploid wheat (Triticum aestivum L.) doubled haploids, mapped with over 700 markers, were tested for three years for ELWL from detached leaf 4 of glasshouse-grown plants. In one experiment, stomata per unit area and leaf thickness parameters from leaf cross-sections were measured. QTLs were identified using QTLCartographer.ResultsELWL was significantly negatively correlated with leaf length, width, area and thickness. Major QTLs for ELWL during 0–3 h and 3–6 h were coincident across trials on 3A, 3B, 4B, 5B, 5D, 6B, 7A, 7B, 7D and frequently coincident (inversely) with leaf size QTLs. Yield in other trials was sometimes associated with ELWL and leaf size phenotypically and genotypically, but more frequently under non-droughted than droughted conditions. QTL coincidence showed only ELWL to be associated with drought/control yield ratio.DiscussionOur results demonstrated that measures of ELWL and leaf size were equally effective predictors of yield, and both were more useful for selecting under favourable than stressed conditions.

【 授权许可】

CC BY