Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites

BMC Research Notes

Rusea Go¹  Parameswari Namasivayam²  Janna Ong Abdullah²  Conie Toh²  Nur Ashikin Psyquay Abdullah³  Ab. Rahim Mohd-Hairul⁴  Nooraini Mohd. Ain⁵  Meilina Ong Abdullah⁶ 

[1] Department of Biology, Faculty of Science, Universiti Putra Malaysia;Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia;Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus;Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang;Institute of Bioscience, Universiti Putra Malaysia;Malaysian Palm Oil Board;

关键词: Fragrance;    Monoterpene synthase;    Morphology;    Orchid;    Stomata;    Trichome;   

DOI  :  10.1186/s13104-017-2872-6

来源: DOAJ

【 摘 要 】

Abstract Background Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the flowers. Results Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites. Next Generation Sequencing (NGS) transcriptomic data of adaxial and abaxial layers of the tissues showed monoterpene synthase transcripts specifically linalool and ocimene synthases distributed throughout the tissues. qPCR analyses taken at different time points revealed high levels of linalool and ocimene synthases transcripts in the early morning with maximal level at 4.00 am but remained low throughout daylight hours. Conclusions Knowledge of the VMP floral anatomy and its fragrance production characteristics, which complemented our previous molecular and biochemical data on VMP, provided additional knowledge on how fragrance and flower morphology are closely intertwined. Further investigation on the mechanisms of fragrance biosynthesis and interaction of potential pollinators would elucidate the evolution of the flower morphology to maximize the reproduction success of this plant.

【 授权许可】

Unknown