Preparation and in vitro release kinetics of ivermectin sustained-release bolus optimized by response surface methodology

PeerJ

article

Ying Gao¹  Lin Peng¹  Hui Ji¹  Dawei Guo¹  Shanxiang Jiang¹  Xiangchun Ruan¹  Xiuge Gao¹ 

[1] College of Veterinary Medicine, Nanjing Agricultural University;College of Animal Science and Technology, Anhui Agricultural University

关键词: Ivermectin;    Bolus;    Dissolution kinetic model;    Korsmeyer–Peppas;    Sustained-release;   

DOI  :  10.7717/peerj.5418

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Sustained-release formulations of ivermectin (IVM) are useful for controlling parasitic diseases in animals. In this work, an IVM bolus made from microcrystalline cellulose (MCC), starch and low-substituted hydroxypropyl cellulose (LS-HPC) was optimized by response surface methodology. The bolus was dissolved in a cup containing 900 mL of dissolution medium at 39.5 °C, under with stirring at 100 rpm. A quadratic model was formulated using analysis of variance according to the dissolution time. The optimized formulation of the bolus contained 8% MCC, 0.5% starch, and 0.25% LS-HPC. The length, width, and height of the prepared IVM bolus were 28.12 ± 0.14, 16.1 ± 0.13, and 13.03 ± 0.05 mm, respectively. The bolus weighed 11.4842 ± 0.1675 g (with a density of 1.95 g/cm3) and contained 458.26 ± 6.68 mg of IVM. It exhibited in vitro sustained-release for over 60 days, with a cumulative amount and percentage of released IVM of 423.72 ± 5.48 mg and 92.52 ± 1.20%, respectively. The Korsmeyer–Peppas model provided the best fit to the dissolution release kinetics, exhibiting an R2 value close to 1 and the lowest Akaike Information Criterion among different models. The parameter n (0.5180) of the Korsmeyer–Peppas model was between 0.45 and 0.89. It was demonstrated that the release mechanism of the IVM bolus followed a diffusive erosion style.

【 授权许可】

CC BY