Coordinative control of G2/M phase of the cell cycle by non-coding RNAs in hepatocellular carcinoma

PeerJ

article

Jun Shi¹  Guangqiang Ye²  Guoliang Zhao²  Xuedong Wang¹  Chunhui Ye²  Keooudone Thammavong²  Jing Xu²  Jiahong Dong¹ 

[1] Department of Hepatobiliary and Pancreas Surgery, Beijing Tsinghua Changguang Hospital, School of Clinical Medicine, Tsinghua University;The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University

关键词: Hepatocellular carcinoma cells;    Microarray;    miRNA;    lncRNA;    mRNA;   

DOI  :  10.7717/peerj.5787

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Objective To investigate the interaction of non-coding RNAs (ncRNAs) in hepatocellular carcinoma. Methods We compared the ncRNAs and mRNAs expression profiles of hepatocellular carcinoma and adjacent tissue by microarray and RT-PCR. The relationship between different ncRNAs and mRNA was analyzed using bioinformatics tools. A regulatory model of ncRNAs in hepatocellular carcinoma cells was developed. Results A total of 1,704 differentially expressed lncRNAs, 57 miRNAs, and 2,093 mRNAs were identified by microarray analyses. There is a co-expression relationship between two ncRNAs (miRNA-125b-2-3p and lncRNA P26302). Bioinformatics analysis demonstrated cyclin-dependent kinases 1 and CyclinA2 as potential targets of miR-125b-2-3p and Polo-like kinase 1 as potential target of lncRNAP26302. All three gene are important components in the G2/M phase of cell cycle. Subsequently real-time polymerase chain reaction (PCR) studies confirmed these microarray results. Conclusion MiR-125b-2-3p and lncRNAP26302 may affect the G2/M phase of the cell cycle through the regulation of their respective target genes. This study shows a role of ncRNAs in pathogenesis of hepatocellular carcinoma at molecular level, providing a basis for the future investigation aiming at early diagnosis and novel treatment of hepatocellular carcinoma.

【 授权许可】

CC BY