Integrative analysis of microRNA and mRNA expression profiles in fetal rat model with anorectal malformation

PeerJ

article

Hui Xiao¹  Rui Huang³  Mei Diao¹  Long Li¹  XiaoDai Cui³ 

[1] Department of Pediatric Surgery, Capital institute of Pediatric;Graduate School of Peking Union Medical College, Chinese Academy of Sciences;Department of Key Laboratory, Capital Institute of Paediatric

关键词: Anorectal malformation;    microRNA;    mRNA;    Fetal rats;   

DOI  :  10.7717/peerj.5774

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundAnorectal malformations (ARMs) are the most common congenital malformation of the gut, and regulated by multiple signal transduction pathways. The microRNA (miRNA) expression profiles and their biologial functions in anorectal malformations (ARMs) remain unclear. The aim of our study was to evaluate miRNA and mRNA expression profiles in the ARM rats.Methods and MaterialsARM was induced with ethylenethiourea (ETU) on gestational day 10. Cesarean deliveries were performed to harvest the embryos on gestional day 20. For the extraction of total RNA, 1 cm terminal hindgut samples were obtained from three fetal rats that had similar weights. The microarrays and quantitative RT-PCR analysis were conducted to evaluate the miRNA and mRNA expression profiles in normal fetal rats (n = 3) and ARM fetal rats (n = 3).ResultsIn total, 33 miRNAs and 772 mRNAs were significantly and differentially expressed in terminal hindgut tissues of ARM group versus control group. Functional annotation was performed to understand the functions and pathways of differentially expressed mRNAs. Also, we constructed a miRNA-target gene regulatory network including 25 differentially expressed miRNAs and 76 mRNAs. Furthermore, the credibility of the microarray-based results were validated by using qRT-PCR.ConclusionThe miRNA and mRNA expression in terminal hindgut tissue of ARM fetal rats might provide a basis for further research on the pathogenesis of ARMs.

【 授权许可】

CC BY