Integrated microRNA and mRNA Expression Profiling Identifies Novel Targets and Networks Associated with Ebstein’s Anomaly

Cells

BasimM. Ayesh¹  Hashim Abdul-Khaliq²  Mohammed Abd El-Rahman²  LeaSimone Becker³  Viktoria Wagner³  Ulrike Fischer³  Eckart Meese³  Masood Abu-Halima³ 

[1] Department of Laboratory Medical Sciences, Alaqsa University, Gaza 4051, Palestine;Department of Pediatric Cardiology, Saarland University Medical Center, 66421 Homburg/Saar, Germany;Institute of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany;

关键词: microRNA;    mRNA;    integration analysis;    congenital heart defect;    Ebstein’s anomaly;   

DOI  :  10.3390/cells10051066

来源: DOAJ

【 摘 要 】

Little is known about abundance level changes of circulating microRNAs (miRNAs) and messenger RNAs (mRNA) in patients with Ebstein’s anomaly (EA). Here, we performed an integrated analysis to identify the differentially abundant miRNAs and mRNA targets and to identify the potential therapeutic targets that might be involved in the mechanisms underlying EA. A large panel of human miRNA and mRNA microarrays were conducted to determine the genome-wide expression profiles in the blood of 16 EA patients and 16 age and gender-matched healthy control volunteers (HVs). Differential abundance level of single miRNA and mRNA was validated by Real-Time quantitative PCR (RT-qPCR). Enrichment analyses of altered miRNA and mRNA abundance levels were identified using bioinformatics tools. Altered miRNA and mRNA abundance levels were observed between EA patients and HVs. Among the deregulated miRNAs and mRNAs, 76 miRNAs (49 lower abundance and 27 higher abundance, fold-change of ≥2) and 29 mRNAs (25 higher abundance and 4 lower abundance, fold-change of ≥1.5) were identified in EA patients compared to HVs. Bioinformatics analysis identified 37 pairs of putative miRNA-mRNA interactions. The majority of the correlations were detected between the lower abundance level of miRNA and higher abundance level of mRNA, except for let-7b-5p, which showed a higher abundance level and their target gene, SCRN3, showed a lower abundance level. Pathway enrichment analysis of the deregulated mRNAs identified 35 significant pathways that are mostly involved in signal transduction and cellular interaction pathways. Our findings provide new insights into a potential molecular biomarker(s) for the EA that may guide the development of novel targeting therapies.

【 授权许可】

Unknown