Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages

PeerJ

article

Abdoallah Sharaf¹  Miroslav Oborník²  Adel Hammad⁴  Sohair El-Afifi⁵  Eman Marei⁵ 

[1] Genetic Department, Faculty of Agriculture, Ain Shams University;Institute of Parasitology, Biology Centre, Czech Academy of Sciences;Faculty of Science, University of South Bohemia;Department of Microbiology, Faculty of Agriculture, Minia University;Department of Agricultural Microbiology, Virology Laboratory, Ain Shams University

关键词: Bacillus megaterium;    Bacteriophage;    Phylogenetic analysis;    Comparative genomics;   

DOI  :  10.7717/peerj.5687

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Next-Generation Sequencing (NGS) technologies provide unique possibilities for the comprehensive assessment of the environmental diversity of bacteriophages. Several Bacillus bacteriophages have been isolated, but very few Bacillus megaterium bacteriophages have been characterized. In this study, we describe the biological characteristics, whole genome sequences, and annotations for two new isolates of the B. megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil samples. Growth analyses indicated that the phages BM5 and BM10 have a shorter latent period (25 and 30 min, respectively) and a smaller burst size (103 and 117 PFU, respectively), in comparison to what is typical for Bacillus phages. The genome sizes of the phages BM5 and BM10 were 165,031 bp and 165,213 bp, respectively, with modular organization. Bioinformatic analyses of these genomes enabled the assignment of putative functions to 97 and 65 putative ORFs, respectively. Comparative analysis of the BM5 and BM10 genome structures, in conjunction with other B. megaterium bacteriophages, revealed relatively high levels of sequence and organizational identity. Both genomic comparisons and phylogenetic analyses support the conclusion that the sequenced phages (BM5 and BM10) belong to different sub-clusters (L5 and L7, respectively), within the L-cluster, and display different lifestyles (lysogenic and lytic, respectively). Moreover, sequenced phages encode proteins associated with Bacillus pathogenesis. In addition, BM5 does not contain any tRNA sequences, whereas BM10 genome codes for 17 tRNAs.

【 授权许可】

CC BY