Gastric Helicobacter pylori infection perturbs human oral microbiota

PeerJ

article

Eng-Guan Chua¹  Ju-Yee Chong¹  Binit Lamichhane¹  K. Mary Webberley¹  Barry J. Marshall¹  Michael J. Wise¹  Chin-Yen Tay¹ 

[1] The Marshall Centre for Infectious Diseases Research and Training, University of Western Australia;Shenzhen Dapeng New District Kuichong People Hospital, Shenzhen City, Guangdong Province;Computer Science and Software Engineering, University of Western Australia

关键词: Oral microbiome;    Urea breath test;    Helicobacter pylori;    16S rRNA sequencing;    Metagenomics;   

DOI  :  10.7717/peerj.6336

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundWe investigated the effects of gastric Helicobacter pylori infection on the daytime and overnight human oral microbiota.MethodsTwenty four volunteers were recruited. Ten tested positive for H. pylori infection by the Carbon-14 Urea Breath Test, and the rest were negative. Two oral swabs were collected: one immediately after waking up in the morning and before brushing teeth, and another in the evening before teeth-brushing. DNA extract acquired from each swab was subjected to Illumina sequencing of 16S rRNA gene amplicons. The microbial abundance and composition were analysed in relation to H. pylori infection status.ResultsHelicobacter pylori-positive individuals had significant changes in the alpha and beta diversities in the daytime samples in comparison to those who were H. pylori negative. To identify which taxa could be significantly affected within the cohorts in the daytime, we employed the LEfSe method. When compared against UBT-negative samples, significantly higher abundances were detected in both Pseudomonas and Roseomonas, while Fusobacterium, Solobacterium, Haemophilus and Streptococcus were significantly decreased in the UBT-positive samples.DiscussionOur data demonstrated that H. pylori infection affects the human daytime oral microbiota. The hitherto undocumented changes of several bacterial genera due to H. pylori infection require more studies to examine their potential health effects on affected individuals.

【 授权许可】

CC BY