Membrane potential (V mem ) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation

PeerJ

article

Mit Balvantray Bhavsar¹  Gloria Cato¹  Alexander Hauschild¹  Liudmila Leppik¹  Karla Mychellyne Costa Oliveira¹  Maria José Eischen-Loges¹  John Howard Barker¹ 

[1] Frankfurt Initiative for Regenerative Medicine, Johann Wolfgang Goethe Universität Frankfurt am Main

关键词: Membrane potential;    Electrical stimulation;    Mesenchymal stem cells;    Osteogenic differentiation;    Cell proliferation;    Vmem;   

DOI  :  10.7717/peerj.6341

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or Vmem. Vmem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation, differentiation and migration, all playing a critical role in embryonic development, healing, and regeneration. Methods With the goal of analyzing the changes in Vmem during cell proliferation and differentiation, here we used direct current electrical stimulation (EStim) to promote cell proliferation and differentiation and simultaneously tracked the corresponding changes in Vmem in adipose derived mesenchymal stem cells (AT-MSC). Results We found that EStim caused increased AT-MSC proliferation that corresponded to Vmem depolarization and increased osteogenic differentiation that corresponded to Vmem hyperpolarization. Taken together, this shows that Vmem changes associated with EStim induced cell proliferation and differentiation can be accurately tracked during these important cell functions. Using this tool to monitor Vmem changes associated with these important cell behaviors we hope to learn more about how these electrochemical cues regulate cell function with the ultimate goal of developing new EStim based treatments capable of controlling healing and regeneration.

【 授权许可】

CC BY