Chemical composition, antioxidant and hepatoprotective activities of methanol extracts from leaves of Terminalia bellirica and Terminalia sericea (Combretaceae)

PeerJ

article

Mansour Sobeh¹  Mona F. Mahmoud²  Rehab A. Hasan³  Mohamed A.O. Abdelfattah⁴  Samir Osman⁵  Harun-or Rashid⁶  Assem M. El-Shazly⁷  Michael Wink¹ 

[1] Institute of Pharmacy and Molecular Biotechnology, Heidelberg University;Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University;Department of Histology, Faculty of Medicine for Girls, Al Azhar University;Department of Science, College of Engineering and Technology, American University of the Middle East;Department of Pharmacognosy, Faculty of Pharmacy, October 6 University;Biotechnology Division, Bangladesh Institute of Nuclear Agriculture Bangladesh;Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University

关键词: Terminalia sericea;    Terminalia bellirica;    HPLC-MS/MS;    Anti-apoptotic;    Hepatoprotection;    Polyphenols;   

DOI  :  10.7717/peerj.6322

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Plants belonging to the genus Terminalia such as Terminalia bellirica and Terminalia sericea are used traditionally to treat several diseases and health disorders. Up to this date, the roots of Terminalia sericea and the fruits of Terminalia bellirica are the mostly studied plant parts. The phytochemical composition and the biological activities of the leaves of both species are not well identified so far. Methods The secondary metabolites of Terminalia bellirica and Terminalia sericea leaves were identified using HPLC-PDA-MS/MS. The antioxidant activities of the leaves extracts were determined by DPPH and FRAP assays. The hepatoprotective potential was evaluated in rats with D-galactosamine induced liver damage. The effect of the extracts on the expression of the anti-apoptotic marker Bcl-2 was measured in an immunohistochemical study. The most abundant compounds identified in the studied extracts were docked into Bcl-2: Bim (BH3) interaction surface using molecular operating environment software. Results A total of 85 secondary metabolites were identified in the leaf extracts of both species. Ellagitannins such as corilagin, chebulagic acid, galloylpunicalagin, and digalloyl-hexahydroxydiphenoyl-hexoside were found to be the major components in Terminalia bellirica whereas flavonoid glycosides including quercetin rutinoside and quercetin galloyl-glucoside were highly abundant in Terminalia sericea. The studied extracts exhibited pronounced antioxidant activities, moderate anti-apoptotic and hepatoprotective potential. In silico docking experiments revealed that the compounds abundant in the extracts were able to bind to Bcl-2: Bim (BH3) interaction surface with an appreciable binding free energy. Discussion The antioxidant and hepatoprotective activities exhibited by the studied extracts might be attributed to the high content of the polyphenols. The anti-apoptotic activity could be due to the interference with the apoptotic pathway mediated by Bcl-2: Bim interaction. These findings support the medicinal relevance of Terminalia bellirica and Terminalia sericea and provide a rational base for their utilization in folk medicine.

【 授权许可】

CC BY