Ultra-performance liquid chromatography-mass spectrometry for precise fatty acid profiling of oilseed crops

PeerJ

article

Alina Chernova¹  Pavel Mazin¹  Svetlana Goryunova¹  Denis Goryunov¹  Yakov Demurin⁶  Lyudmila Gorlova⁶  Anna Vanyushkina¹  Waltraud Mair¹  Nikolai Anikanov¹  Ekaterina Yushina¹  Anna Pavlova¹  Elena Martynova¹  Sergei Garkusha⁸  Zhanna Mukhina⁸  Elena Savenko⁸  Philipp Khaitovich¹ 

[1] Center of Life Sciences, Skolkovo Institute of Science and Technology;Faculty of Computer Science, National Research University Higher School of Economics;Institute for Information Transmission Problems ,(Kharkevich Institute), Russian Academy of Sciences;Institute of General Genetics, Russian Academy of Sciences;Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University;Pustovoit All-Russia Research Institute of Oil Crops;Pirogov Russian National Research Medical University;All-Russia Rice Research Institute

关键词: Ultra-performance liquid chromatography-mass spectrometry;    Oil crops;    Rapeseed;    Fatty acids;    Breeding;    Gas chromatography-flame ionization detection;    Sunflower;   

DOI  :  10.7717/peerj.6547

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Oilseed crops are one of the most important sources of vegetable oils for food and industry. Nutritional and technical properties of vegetable oil are primarily determined by its fatty acid (FA) composition. The content and composition of FAs in plants are commonly determined using gas chromatography-mass spectrometry (GS-MS) or gas chromatography-flame ionization detection (GC-FID) techniques. In the present work, we applied ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) technique to FA profiling of sunflower and rapeseed seeds and compared this method with the GC-FID technique. GC-FID detected 11 FAs in sunflower and 13 FAs in rapeseed, while UPLC-MS appeared to be more sensitive, detecting about 2.5 times higher numbers of FAs in both plants. In addition to even-chain FAs, UPLC-MS was able to detect odd-chain FAs. The longest FA detected using GC-FID was an FA with 24 carbon atoms, whereas UPLC-MS could reveal the presence of longer FAs with the tails of up to 28 carbon atoms. Based on our results, we may conclude that UPLC-MS has great potential to be used for the assessment of FA profiles of oil crops.

【 授权许可】

CC BY