The effect of platelet lysate in culture of PDLSCs: an in vitro comparative study

PeerJ

article

Duaa A. Abuarqoub¹  Nazneen Aslam¹  Raghda B. Barham¹  Nidaa A. Ababneh¹  Diana A. Shahin¹  Abdallah A. Al-oweidi¹  Hanan D. Jafar¹  Mazin A. Al-Salihi¹  Abdalla S. Awidi¹ 

[1] Cell Therapy Center, The University of Jordan;School of Medicine, The University of Jordan

关键词: Enzymatic digestion;    PL;    Explant;    FBS;    PDLSCs;    Osteogenic differentation;   

DOI  :  10.7717/peerj.7465

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCellular therapy clinical applications require large-scale production of stem cells. Therefore, abundance, ease of isolation, and proliferative potential are the most important factors in choosing the appropriate source of cells for transplantation studies. Multipotent stem cells obtained from periodontal ligament (PDL) can be used in periodontal tissue regeneration. In this study, we aimed to evaluate and compare the characteristics of periodontal ligament stem cells (PDLSCs), extracted by either enzymatic digestion or explant methods, and expanded using two different serum types: fetal bovine serum (FBS) and xeno-free platelet lysate (PL).MethodsExpanded PDLSCs were assessed for their proliferation capacity, surface markers expression, colony formation, differentiation potential and ability to self-renewal. Most importantly, PDLSCs were evaluated for their ability to produce osteoblasts in vitro.ResultsPDLSCs isolated by explant method and expanded in PL serve as a promising source of stem cells for osteoblasts regeneration. These cells showed higher proliferation capacity, they retained their stemness characteristics throughout the passages and they revealed an increase in the expression level of osteogenic markers, without showing any karyotypic abnormalities after cell expansion.ConclusionsPDLSCs produced using explant extraction method and expanded in cell culture media supplemented with PL provide an excellent source of xeno-free cells for the generation of functional osteoblasts.

【 授权许可】

CC BY