Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments

Advances in Horticultural Science

Gholamreza Abdi¹  Morteza Khush-hkhui²  Akhtar Shekafandeh² 

[1] Department of Biotechnology, Persian GulfResearch Institute, Persian Gulf University, Bushehr, 7516913817, Iran;Department of Horticulture, College of Agriculture, Shiraz University, Shiraz, Iran;

关键词: Explant;    In vitro;    Glutamic Acid;    MS medium;   

DOI  :  10.13128/ahs-24090

来源: DOAJ

【 摘 要 】

Abstract:A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.) using the leaf segments. Direct somatic embryogenesis were induced using by half-strength MS medium supplemented with 2,4-D (0.5 mg·l-1), glutamic acid (100 mg·l-1), 4% sucrose and 8 g·l-1 agar. Germinated embryo to form plantlets enhanced on MS medium supplemented with NAA (0.1 mg·l-1) and Kin (2 mg·l-1). Regenerated plants with well-developed root and shoot systems were successfully (72%) transferred to greenhouse.

【 授权许可】

Unknown