Comprehensive classification of the plant non-specific lipid transfer protein superfamily towards its sequence–structure–function analysis

PeerJ

article

Jean-François Dufayard¹  Gilles Labesse²  Manuel Ruiz¹  Frédéric de Lamotte¹  Cécile Fleury¹  Jérôme Gracy²  Marie-Françoise Gautier¹  Jean-Luc Pons² 

[1] UMR AGAP;CBS

关键词: nsLTP;    Plant;    Phylogeny;    Molecular modeling;    Structure–function relationships;    Multigenic family;    Functional annotation;    Homology modeling;   

DOI  :  10.7717/peerj.7504

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Non-specific Lipid Transfer Proteins (nsLTPs) are widely distributed in the plant kingdom and constitute a superfamily of related proteins. Several hundreds of different nsLTP sequences—and counting—have been characterized so far, but their biological functions remain unclear. It has been clear for years that they present a certain interest for agronomic and nutritional issues. Deciphering their functions means collecting and analyzing a variety of data from gene sequence to protein structure, from cellular localization to the physiological role. As a huge and growing number of new protein sequences are available nowadays, extracting meaningful knowledge from sequence–structure–function relationships calls for the development of new tools and approaches. As nsLTPs show high evolutionary divergence, but a conserved common right handed superhelix structural fold, and as they are involved in a large number of key roles in plant development and defense, they are a stimulating case study for validating such an approach. Methods In this study, we comprehensively investigated 797 nsLTP protein sequences, including a phylogenetic analysis on canonical protein sequences, three-dimensional structure modeling and functional annotation using several well-established bioinformatics programs. Additionally, two integrative methodologies using original tools were developed. The first was a new method for the detection of (i) conserved amino acid residues involved in structure stabilization and (ii) residues potentially involved in ligand interaction. The second was a structure–function classification based on the evolutionary trace display method using a new tree visualization interface. We also present a new tool for visualizing phylogenetic trees. Results Following this new protocol, an updated classification of the nsLTP superfamily was established and a new functional hypothesis for key residues is suggested. Lastly, this work allows a better representation of the diversity of plant nsLTPs in terms of sequence, structure and function.

【 授权许可】

CC BY