Invasions of gladiolus rust in North America are caused by a widely-distributed clone of Uromycestransversalis

PeerJ

article

Jeffery A. DeLong¹  Jane E. Stewart¹  Alberto Valencia-Botín³  Kerry F. Pedley⁴  James W. Buck¹  Marin T. Brewer¹ 

[1] Department of Plant Pathology, University of Georgia;Department of Bioagricultural Sciences and Pest Management, Colorado State University;Centro Universitario de la Ciénega, Universidad de Guadalajara;Foreign Disease-Weed Science Research Unit, United States Department of Agriculture Agricultural Research Service

关键词: Clonal population;    Invasion;    Ornamental rust;    Whole genome sequencing;    Genetic diversity;    Microsatellites;   

DOI  :  10.7717/peerj.7986

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Uromyces transversalis, the causal agent of Gladiolus rust, is an invasive plant pathogen in the United States and is regulated as a quarantine pathogen in Europe. The aim of this research was to: (i) determine the origin of introductions of U. transversalis to the United States, (ii) track the movement of genotypes, and (iii) understand the worldwide genetic diversity of the species. To develop molecular markers for genotyping, whole genome sequencing was performed on three isolates collected in the United States. Genomes were assembled de novo and searched for microsatellite regions. Primers were developed and tested on ten isolates from the United States resulting in the identification of 24 polymorphic markers. Among 92 isolates collected from Costa Rica, Mexico, New Zealand, Australia, and the United States there were polymorphisms within isolates with no genotypic diversity detected among isolates; however, missing data among the New Zealand and Australia isolates due to either poor amplification of degraded DNA or null alleles as a result of genetic differences made it difficult to generate conclusions about these populations. The microsatellite loci and flanking regions showed high diversity and two divergent genomes within dikaryotic individuals, yet no diversity among individuals, suggesting that the invasive U. transversalis populations from North America are strictly clonal.

【 授权许可】

CC BY