Comparison of bacterial diversity and abundance between sexes of Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) from China

PeerJ

article

Chunhui Guo¹  Xin Peng¹  Xialin Zheng²  Xiaoyun Wang²  Ruirui Wang¹  Zongyou Huang²  Zhende Yang¹ 

[1] College of Forestry, Guangxi University;Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, College of Agriculture, Guangxi University;Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University

关键词: Leptocybe invasa;    16S rRNA;    High throughput sequencing;    Bacteria;    Diversity;   

DOI  :  10.7717/peerj.8411

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundInsects harbor a myriad of microorganisms, many of which can affect the sex ratio and manipulate the reproduction of the host. Leptocybe invasa is an invasive pest that causes serious damage to eucalyptus plantations, and the thelytokous parthenogenesis, low temperature resistance, protection in galls, generation overlap and small body of L. invasa contribute to its rapid invasion and population growth. However, the endosymbiotic bacterial composition, abundance and sex differences of L. invasa remain unclear. Therefore, this research aimed to identify the bacterial communities in L. invasa adults and compare them between the sexes of L. invasa lineage B.ResultsThe Illumina MiSeq platform was used to compare bacterial community composition between females and males of L. invasa by sequencing the V3–V4 region of the 16S ribosomal RNA gene. A total of 1,320 operational taxonomic units (OTUs) were obtained. These OTUs were subdivided into 24 phyla, 71 classes, 130 orders, 245 families and 501 genera. At the genus level, the dominant bacteria in females and males were Rickettsia and Rhizobium, respectively.ConclusionThe endosymbiotic bacteria of L. invasa females and males were highly diverse. There were differences in the bacterial community of L. invasa between sexes, and the bacterial diversity in male specimens was greater than that in female specimens. This study presents a comprehensive comparison of bacterial communities in L. invasa and these data will provide an overall view of the bacterial community in both sexes of L. invasa with special attention on sex-related bacteria.

【 授权许可】

CC BY