AIF1 was identified as an up-regulated gene contributing to CSFV Shimen infection in porcine alveolar macrophage 3D4/21 cells

PeerJ

article

Xiaocheng Gong¹  Xuepeng Li¹  Xin You¹  Aoxue Hu¹  Min Liu¹  Huimin Yao¹  Jun He¹  Xianghan Zhang¹  Pengbo Ning¹ 

[1] School of Life Science and Technology, Xidian University;Engineering Research Center of Molecular & Neuroimaging, Ministry of Education

关键词: CSFV Shimen;    AIF1;    IL6;    Porcine alveolar macrophages;   

DOI  :  10.7717/peerj.8543

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Classical swine fever (CSF) is a disease that is characterized by diffuse hemorrhaging, high fever, and high mortality rates. The pro-inflammatory characteristics of allograft inflammatory factor 1 (AIF1) have been well documented; however, insufficient attention has been given to porcine AIF1. In the present study, AIF1 was identified as a key player contributing to CSFV Shimen infection in porcine alveolar macrophage (PAM) 3D4/21 cell line. Our evaluation showed that AIF1 mRNA and protein are expressed at a time-dependent high level in CSFV Shimen-infected PAM 3D4/21 cells. The transcription and translation of IL6 were also significantly upregulated in infected PAM 3D4/21 cells. By utilizing overexpression RNAs approach, we showed that the cellular AIF1 induced an increased IL6 in PAM 3D4/21 cells. Furthermore, silencing of AIF1 suppressed CSFV Shimen-induced IL6 production in PAM 3D4/21 cells and also inhibited CSFV replication, whereas overexpression of recombinant AIF1 was beneficial for the replication of CSFV Shimen and promoting IL6 production in CSFV Shimen-infected PAM 3D4/21 cells. It is suggested CSFV Shimen induced IL6 in PAM 3D4/21 cells via AIF1 activation, which help clarify the AIF1-related inflammatory processes that occur on CSFV Shimen infected macrophages.

【 授权许可】

CC BY