Rhizosphere bacterial and fungal communities during the growth of Angelica sinensis seedlings cultivated in an Alpine uncultivated meadow soil

PeerJ

article

Zhengjun Chen¹  Zhigang An¹  Fengxia Guo¹  Yuan Chen¹  Gang Bai¹ 

[1] College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University;Pharmacy Department, Gansu University of Chinese Medicine;Gansu Engineering Lab of Resource Reservation and Utilization for Characteristic Chinese Medicine, Gansu Tasly Zhongtian Pharmaceutical Co., Ltd.

关键词: Angelica sinensis;    Seedlings;    Rhizosphere;    Bacteria;    Fungi;    Diversity;    Community function;   

DOI  :  10.7717/peerj.8541

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Angelica sinensis seedlings are grown in alpine uncultivated meadow soil with rainfed agroecosystems to ensure the quality of A. sinensis after seedling transplantation. The aim was to investigate the rhizosphere bacterial and fungal communities during the growth stages of A. sinensis seedlings. Methods The bacterial and fungal communities were investigated by HiSeq sequencing of 16S and 18S rDNA, respectively. Results Proteobacteria and Bacteroidetes were bacterial dominant phyla throughout growth stages. Fungal dominant phyla varied with growth stages, dominant phyla Ascomycota and Chytridiomycota in AM5, dominant phyla Basidiomycota, Ascomycota and Zygomycota in BM5, and dominant phyla Basidiomycota and Ascomycota in CM5. There was no significant variation in the alpha-diversity of the bacterial and fungal communities, but significant variation was in the beta-diversity. We found that the variation of microbial community composition was accompanied by the changes in community function. The relative abundance of fungal pathogens increased with plant growth. We also identified the core microbes, significant-changing microbes, stage-specific microbes, and host-specific microbes. Plant weight, root length, root diameter, soil pH, rainfall, and climate temperature were the key divers to microbial community composition. Conclusions Our findings reported the variation and environmental drivers of rhizosphere bacterial and fungal communities during the growth of A. sinensis seedlings, which enhance the understanding of the rhizosphere microbial community in this habitat.

【 授权许可】

CC BY